How does the ubiquitin-like protein NEDD8 activate ubiquitin ligase machineries?

Linkage-specific ubiquitin chain formation depends on a lysine hydrocarbon ruler (opens in new window)

Author(s): Joanna Liwocha, David T. Krist, Gerbrand J. van der Heden van Noort, Fynn M. Hansen, Vinh H. Truong, Ozge Karayel, Nicholas Purser, Daniel Houston, Nicole Burton, Mark J. Bostock, Michael Sattler, Matthias Mann, Joseph S. Harrison, Gary Kleiger, Huib Ovaa, Brenda A. Schulman
Published in: Nature Chemical Biology, Issue 17/3, 2021, Page(s) 272-279, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-020-00696-0

K6-linked ubiquitylation marks formaldehyde-induced RNA-protein crosslinks for resolution. (opens in new window)

Author(s): Aldwin Suryo Rahmanto, Christian J Blum, Claudia Scalera , Jan B Heidelberger, Mikhail Mesitov , Daniel Horn-Ghetko, Justus F Gräf, Ivan Mikicic, Rebecca Hobrecht, Anna Orekhova, Matthias Ostermaier, Stefanie Ebersberger, Martin M Möckel, Nils Krapoth, Nádia Da Silva Fernandes, Athanasia Mizi, Yajie Zhu, Jia-Xuan Chen, Chunaram Choudhary, Argyris Papantonis, Helle D Ulrich, Brenda A Schulman,
Published in: Molecular Cell, Issue 83/23, 2023, Page(s) 4272-4289.e10, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2023.10.011

CUL5-ARIH2 E3-E3 ubiquitin ligase structure reveals cullin-specific NEDD8 activation (opens in new window)

Author(s): Sebastian Kostrhon, J. Rajan Prabu, Kheewoong Baek, Daniel Horn-Ghetko, Susanne von Gronau, Maren Klügel, Jérôme Basquin, Arno F. Alpi, Brenda A. Schulman
Published in: Nature Chemical Biology, Issue 17/10, 2021, Page(s) 1075-1083, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-021-00858-8

Structure of CRL7FBXW8 reveals coupling with CUL1-RBX1/ROC1 for multi-cullin-RING E3-catalyzed ubiquitin ligation. (opens in new window)

Author(s): Hopf LVM, Baek K, Klügel M, Gronau Sv, Xiong Y, Schulman BA
Published in: Nature Structural and Molecular Biology, Issue 29, 2022, Page(s) 854-864, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-022-00815-6

Decoding the messaging of the ubiquitin system using chemical and protein probes (opens in new window)

Author(s): Lukas T. Henneberg, Brenda A. Schulman
Published in: Cell Chemical Biology, Issue 28/7, 2021, Page(s) 889-902, ISSN 2451-9456
Publisher: Elsevier
DOI: 10.1016/j.chembiol.2021.03.009

Activity-based profiling of cullin-RING ligase networks by conformation-specific probes. (opens in new window)

Author(s): Henneberg LT, Singh J, Duda DM, Baek K, Yanishevski D, Murray PJ, Mann M, Sidhu SS, Schulman BA.
Published in: Nature Chemical Biology, 2023, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-023-01392-5

NEDD8 and ubiquitin ligation by cullin-RING E3 ligases (opens in new window)

Author(s): Kheewoong Baek, Daniel C Scott, Brenda A Schulman
Published in: Current Opinion in Structural Biology, Issue 67, 2021, Page(s) 101-109, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2020.10.007

Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity (opens in new window)

Author(s): Donghyuk Shin, Rukmini Mukherjee, Diana Grewe, Denisa Bojkova, Kheewoong Baek, Anshu Bhattacharya, Laura Schulz, Marek Widera, Ahmad Reza Mehdipour, Georg Tascher, Paul P. Geurink, Alexander Wilhelm, Gerbrand J. van der Heden van Noort, Huib Ovaa, Stefan Müller, Klaus-Peter Knobeloch, Krishnaraj Rajalingam, Brenda A. Schulman, Jindrich Cinatl, Gerhard Hummer, Sandra Ciesek, Ivan Dikic
Published in: Nature, Issue 587/7835, 2020, Page(s) 657-662, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-020-2601-5

E3 ligase autoinhibition by C-degron mimicry maintains C-degron substrate fidelity. (opens in new window)

Author(s): Daniel C Scott, Moeko T King, Kheewoong Baek, Clifford T Gee, Ravi Kalathur, Jerry Li, Nicholas Purser, Amanda Nourse, Sergio C Chai, Sivaraja Vaithiyalingam, Taosheng Chen, Richard E Lee, Stephen J Elledge, Gary Kleiger, Brenda A Schulman.
Published in: Molecular Cell, Issue 83/5, 2023, Page(s) 770-786.e.9, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2023.01.019

NEDD8 nucleates a multivalent cullin–RING–UBE2D ubiquitin ligation assembly (opens in new window)

Author(s): Kheewoong Baek, David T. Krist, J. Rajan Prabu, Spencer Hill, Maren Klügel, Lisa-Marie Neumaier, Susanne von Gronau, Gary Kleiger, Brenda A. Schulman
Published in: Nature, Issue 578/7795, 2020, Page(s) 461-466, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-020-2000-y

Systemwide disassembly and assembly of SCF ubiquitin ligases. (opens in new window)

Author(s): Baek K, Scott DC, Henneberg LT, King M, Mann M, Schulman BA
Published in: Cell, Issue 186/9, 2023, Page(s) 1895-1911.e.21, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2023.02.035

New classes of E3 ligases illuminated by chemical probes. (opens in new window)

Author(s): Daniel Horn-Ghetko and Brenda A. Schulman
Published in: Current Opinion in Structural Biology, Issue 73, 2022, Page(s) 102341, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2022.102341

How the ends signal the end: Regulation by E3 ubiquitin ligases recognizing protein termini. (opens in new window)

Author(s): Sherpa, D., Chrustowicz, J., & Schulman, B.
Published in: Molecular Cell, Issue 82/8, 2022, Page(s) 1424-1438, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2022.02.004

A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transporters. (opens in new window)

Author(s): Langlois CR, Beier V, Karayel O, Chrustowicz J, Sherpa D, Mann M, Schulman BA.
Published in: EMBO Reports, Issue 23, 2022, Page(s) e53835, ISSN 1469-221X
Publisher: Nature Publishing Group
DOI: 10.15252/embr.202153835

Ubiquitin ligation to F-box protein targets by SCF–RBR E3–E3 super-assembly (opens in new window)

Author(s): Daniel Horn-Ghetko, David T. Krist, J. Rajan Prabu, Kheewoong Baek, Monique P. C. Mulder, Maren Klügel, Daniel C. Scott, Huib Ovaa, Gary Kleiger, Brenda A. Schulman
Published in: Nature, Issue 590/7847, 2021, Page(s) 671-676, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-021-03197-9

An expanded lexicon for the ubiquitin code. Nature Reviews Molecular Cell Biology. doi. (opens in new window)

Author(s): Dikic, I., & Schulman, B. A.
Published in: Nature Reviews Molecular Cell Biology, Issue 24/4, 2022, Page(s) 273-287, ISSN 1471-0072
Publisher: Nature Publishing Group
DOI: 10.1038/s41580-022-00543-1

Structural snapshots along K48-linked ubiquitin chain formation by the HECT E3 UBR5. (opens in new window)

Author(s): Hehl LA, Horn-Ghetko D., Prabu JR, Vollrath R., Vu DT, Pérez Berrocal DA, Mulder MPC, van der Heden van Noort GJ, Schulman BA.
Published in: Nature Chemical Biology, 2023, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-023-01414-2

A pro-Fluorescent Ubiquitin-based Probe to Monitor Cysteine-based E3 Ligase Activity. (opens in new window)

Author(s): Pérez Berrocal D, Vishwanatha T, Horn Ghetko D, Bosch J, Hehl L, Kostrhon S, Misra M, Dikic I, Geurink P, van Dam H, Schulman B, Mulder MPC.
Published in: Angewandte Chemie International Edition, 2023, Page(s) e202303319, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202303319